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Which of the following statements is incorrect regarding polyadenylation of a eukaryotic mRNA a. The CPSF...

Which of the following statements is incorrect regarding polyadenylation of a eukaryotic mRNA

a. The CPSF and CstF proteins are involved in polyadenylation

b. The mRNA is cut prior to polyadenylation

c. The adenines are added by poly(A) polymerase

d. The poly(A) tail is a series of up to 250 adenine nucleotides that are placed at the 5’ end of the RNA

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Answer #1

Option D

Option D is incorrect because tailing/polyadenylation occurs at 3' end of mRNA.

RNA processing involves 3 steps -

1. Capping - addition of 7 methyl guanosine at 5' end by capping enzyme.

2. Tailing - addition of polyadenine tail at 3' end by polyA polymerase enzyme. The mRNA before tailing is cut at polyadenylation site by CStF and CPSF.

Both capping and tailing are important for the stabilization of messenger RNA, help it to initiate translation and also protect it against degradation by cytoplasmic nucleases.

3. Splicing - removal of introns and joining of exons.

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