Question

Optical density of turbidity measured using a spectrophotometer is dependent on what 2 things? Please explain.

Answer 1

The optical density of turbidity measured by Spectrophotometer dependent on

1. Concentration of the solution

2.  Length / thickness of the solution

Spectrophotometer works on the principle based on the Beer-Lambert's law

Beer-Lambert's law states that, the amount of light absorbed is directly proportional to the concentration of solute and length and thickness of the the solution

ie

A ∝ CL

Where,

A = Absorbance / Optical density of solution

C = Concentration of solution

L= Path length

or, A = KCL

K = Absorption coefficient, C = Concentration of solution and L = Path Length