Question

Which type of vertebrate tissue would you expect to be an excellent source of tubulin? of actin? of keratin? Which protein would you expect to be the least soluble and most difficult to extract? What types of protein would you expect as contaminants in a preparation of tubulin? Which in a preparation of actin?

Answer 1

-Brain, muscles and skin expect to be an excellent source of tubulin, actin and keratin.

-Keratin protein is expect to be the least soluble and more difficult to extract.

-Tropomyosin, troponin or other actin binding proteins are expected as contaminants in a preparation of tubulin.

-Capping protein like F actin capping protein is expected to be contaminants in preparation of actin.

This capping protein access to the free barbed ends of actin filament and is therefore a major factors affecting actin filament elongation.