Question

I identified a new histone modification in my laboratory. How do I determine all sites on...

I identified a new histone modification in my laboratory. How do I determine all sites on the human genome where this modification is present?

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Answer #1

The method for analysing histone modification in genome is chromatin immunoprecipitaion (chIP) based method. The principle for the method is antigen -antibody reaction. It mainly based on the specificity of antibody. The antibodies will contact with the protein or modified specific site along withany bound DNA. Almost 9 types of histone modification has identified and among this methylation, phosphorylation, Acetylation and ubiquitylatiin are most studied one.Gene modification in different sites ( specific loci) on human genome can be identified by chIP method and this method utilizing the specificity of antibody to detect the modified site of genome.

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