I identified a new histone modification in my laboratory. How do I determine all sites on the human genome where this modification is present?
The method for analysing histone modification in genome is chromatin immunoprecipitaion (chIP) based method. The principle for the method is antigen -antibody reaction. It mainly based on the specificity of antibody. The antibodies will contact with the protein or modified specific site along withany bound DNA. Almost 9 types of histone modification has identified and among this methylation, phosphorylation, Acetylation and ubiquitylatiin are most studied one.Gene modification in different sites ( specific loci) on human genome can be identified by chIP method and this method utilizing the specificity of antibody to detect the modified site of genome.
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