Question

A student tried to anneal a single-stranded oligonucleotide to a complementary ssDNA. It didn't work!! Maybe...

A student tried to anneal a single-stranded oligonucleotide to a complementary ssDNA. It didn't work!! Maybe next time she should try

Select one:

a. adding more salt

b. adding formamide

c. lowering the temperature

d. a and b

e. a and c

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

The correct option is : C

Lowering of temperature.

It is because when the temperature is lowered the hydrogen bonds formed between the complementary bases get stabilized as we know that increase in temperature decreases the bond strength.

Hydrogen bond being weak non covalent interaction is sensitive to temperature.

Other options are incorrect because increasing nthe salt concentration not helps in anealing but in denaturation of the DNA.

Formamide also decreases the stability of double stranded DNA hence it will also decrease chances of anealing.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
What are standard conditions of ΔH° for the data you will use? Temperature 0 K 100...
What are standard conditions of ΔH° for the data you will use? Temperature 0 K 100 K 273 K 298 K Pressure 1.0 Pa 100 Pa 1.0 bar 100 bar Concentration 1.0 g/L 1.0 mol/L 1.0 mg/L mol/kg Select the complete reactions (including phases) for the reaction between solid NaOH and a solution of HCl. 2NaOH(s) + HCl(aq) → 2NaCl(aq) + H2O(l) NaOH(s) + HCl(aq) → NaCl(aq) + H2O(l) NaOH(s) + HCl(aq) → NaCl(s) + H2O(l) Calculate the theoretical ΔH°r...
SECTION A: READ THE PASSAGE AND ANSWER QUESTIONS 1-3 Emma, a monthly salaried worker, received 1,300...
SECTION A: READ THE PASSAGE AND ANSWER QUESTIONS 1-3 Emma, a monthly salaried worker, received 1,300 cedis as her net salary; she heard that this year’s inflation is at 18%. This situation caused prices to soar so high that she complained about everything she buys. In the previous year she was able to buy herself, dresses, a basket of foodstuffs, a small bag of oranges, a dozen of drinks and her transportation. However, this year, she couldn’t afford the full...
1. Entrepreneurship: Urban Escape case study Watch the video in which Maia Josebachvili and Bram Levy...
1. Entrepreneurship: Urban Escape case study Watch the video in which Maia Josebachvili and Bram Levy discuss starting Urban Escapes, which has since been bought by LivingSocial. Then use your knowledge of entrepreneurship to answer the questions. Video Script: >> So my dad once told me that the recipe for success is hard work, intelligence, and luck. I'm Maia, founder of Urban Escapes, and now National Director of Living Social Escapes. So after college I actually did the thing that...
The project has been performing as planned, and the team members have been exhibiting increasing levels...
The project has been performing as planned, and the team members have been exhibiting increasing levels of trust between each other. The sponsor and the buyer have been satisfied with the delivery of business value, and user acceptance testing results are positive. A technical expert consulting with the team has expressed concerns that the team is not in agreement on the best way to deliver to the next review. If the project manager wants to support the team in reaching...
The Martins (Mack and Minnie) own a lovely house in rural BC. One summer, they hired...
The Martins (Mack and Minnie) own a lovely house in rural BC. One summer, they hired two local young people (Tad and Shamir) to help with some landscaping. Tad was especially impressed when he saw the Martin property and asked if he could hang out there from time to time. Mack and Minnie explained that they valued their privacy, and that Tad and Shamir could only attend on the property in order to effect the landscaping work. The day after...
Question 1 Which need in Maslow's hierarchy reflects a desire for love, friendship, affiliation, and group...
Question 1 Which need in Maslow's hierarchy reflects a desire for love, friendship, affiliation, and group acceptance? A.      Safety B.      Self-actualization C.      Belongingness D.      Esteem Question 2 If a person does not feel accepted or loved, they have not reached what level, according to Maslow ? A.      Level 1 B.      Level 5 C.      Level 3 D.      Level 2 Question 3 What stage of Maslow’s pyramid do you think could be skipped to move to another level ? A.      Psychological needs...
QUESTION 1 Which one of the following would NOT be necessary for an offer to have...
QUESTION 1 Which one of the following would NOT be necessary for an offer to have legal standing? A. The language must reflect the intent to become a party to a contract. B. All of the conditions under which the offer would be terminated must be identified. C. All the significant terms and/or conditions must be contained in the offer. D. The offer must be effectively communicated to the other party. 3 points    QUESTION 2 Which one of the...
The Business Case for Agility “The battle is not always to the strongest, nor the race...
The Business Case for Agility “The battle is not always to the strongest, nor the race to the swiftest, but that’s the way to bet ’em!”  —C. Morgan Cofer In This Chapter This chapter discusses the business case for Agility, presenting six benefits for teams and the enterprise. It also describes a financial model that shows why incremental development works. Takeaways Agility is not just about the team. There are product-management, project-management, and technical issues beyond the team’s control. Lean-Agile provides...
Pandora is the Internet’s most successful subscription radio service. As of June 2013, it had over...
Pandora is the Internet’s most successful subscription radio service. As of June 2013, it had over 200 million registered users (140 million of which access the service via a mobile device) and over 70 million active listeners. Pandora now accounts for more than 70% of all Internet radio listening hours and a 7% share of total U.S. radio listening (both traditional and Internet). At Pandora, users select a genre of music based on a favorite musician, and a computer algorithm...
Muffler Magic* Muffler Magic is a fast-growing chain of 25 automobile service centers in Nevada. Originally...
Muffler Magic* Muffler Magic is a fast-growing chain of 25 automobile service centers in Nevada. Originally started 20 years ago as a muffler repair shop by Ronald Brown, the chain expanded rapidly to new locations, and as it did so Muffler Magic also expanded the services it provided, from muffler replacement to oil changes, brake jobs, and engine repair. Today, one can bring an automobile to a Muffler Magic shop for basically any type of service, from tires to mufflers...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT