Question

Briefly describe 5 approaches you might use to specifically inhibit the function of a protein in a cellular signaling pathway

Answer 1

Five specific approaches to inhibit the function of a protein in a cellular signaling pathway:

i. Generate a loss of function mutant of the protein

ii. Generate a siRNA against the specific protein-coding transcript

iii. Develop a specific drug/inhibitor against the protein function

iv. Overexpress a protein that sequesters the given protein so that it is no longer available for its function

v. Overexpress a protein that increases the degradation of given protein so that it is no longer available for its function

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