Question

You discover a novel virus A in bats. You would like to isolate the virus, sequence it, and grow it up in the lab so that diagnostic assays may be developed, and vaccines can be generated.

1. In order to grow up the virus, you need permissive cells. What is the first piece of information you will need before you begin identifying permissive cells to grow virus A in the lab?
2. Sequencing information reveals that the virus is a variant of the measles virus. Given this, how would that influence the cells you select for growing up the virus?
3. "You have identified the right culture conditions and are successfully growing up virus, but you make a mistake an add a potent inhibitor of cellular metabolism to the viral culture. Briefly describe how this will impact virus growth and why?"
4. If this virus was an ssRNA virus and mutated to evade detection by your immune system, which toll-like receptor would it need to evade?

Answer 1

The most important thing to be noted while selecting permissive cells for growing viruses is that the cells should have surface proteins that helps the viruses to go inside the cell. The most important thing to be noted while selecting permissive cells for growing viruses is that the cells should have surface proteins that helps the viruses to go inside the cell.The most important thing to be noted while selecting permissive cells for growing viruses is that the cells should have surface proteins that helps the viruses to go inside the cell.