Question

If someone has one copy of HbA and one of HbS, what symptoms would they have?

Why didn’t we have to stain the proteins in the native gel used in this lab?

Which hemoglobin ran further (more towards the positive end) in the gel? Why?

Why was this procedure called “native” gel electrophoresis?

Answer 1

• Person having both copy of allele i.e, HbA/HbS show sickel cell trait because sickle cell mutation is dominant mutation that a inidividual show.
• Native gels do not not stained because if your protein needs to be studied in situ or eluted from the gel while maintaining the native form. gel staining procedure will genrally fix (mainly denature) the protein in the gel so that the subsequent staining can bind the protein, allowing protein's visualization in the gel.
• HbA (healthy) heamoglobin ran further in the gel becauase HbA is more negative due to glutamate than HbS which is less negative due to the substitution to valine amino acid.
• Because in native gel we dont give SDS treatment (reducing treatment for denaturation) and studied the protein in native form without denaturation