Question

1. List 4 things required for tRNA charging 2. How many aminoacyl-tRNA synthetases are there are...

1. List 4 things required for tRNA charging

2. How many aminoacyl-tRNA synthetases are there are cells AND why that specific number?

3. In prokaryotes, what is the sequence element that is upstream of the start codon and required for efficient translation called?

4. In eukaryotes, what is the sequence element upstream of the start codon that is required for efficient translation called?

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Answer #1

1. In tRNA charging, specific and appropriate amino acid is bind to transfer RNA (tRNA). tRNA charging requires-

aminoacyl-tRNA synthetase

amino acid

ATP

tRNA

2. There are twenty ainoacyl-tRNA synthetase exists. As there is twenty amino acids, each synthetase is responsible for each amino acid.

3- Shine-Dalgarno Sequence-this is located upstream of the start codon, around 8 bases and this enzyme is used to help the ribosome to get attached with the mRNA. This steps helps to start orl initiate translation.

4. 5? untranslated region or 5? UTR,located upstream of the start codon. This region will help to control translation.

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