Question

what is the effect of porphyrin and ethidium on BSSH II activity?

what is the effect of porphyrin and ethidium on BSSH II activity?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

BssHII is a restriction enzyme. Porphyrin is a heterocyclic macrocycle organic compounds and in its presence it acts as catalyst in the process of DNA cutting. It help in boosting the function of the BssHII restriction enzyme in fragmenting the DNA into desired fragments. The ethidium is responsible for causing a reduction in the mobility of DNA fragments. Since it slows down the mobility being an intercalating agent it acts as fluorescence and helps with detection of the movement of the fragments.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
How does ethidium bromide effect the melting temperature of DNA?
How does ethidium bromide effect the melting temperature of DNA?
What is the purpose of ethidium bromide in agarose gel electrophoresis?
What is the purpose of ethidium bromide in agarose gel electrophoresis?
what purpose does the ethidium bromide serve? why should you always avoid contact with this compound?
what purpose does the ethidium bromide serve? why should you always avoid contact with this compound?
c) A final step in the analysis of enzymatic activity during Enzyme Practical I: Effect of...
c) A final step in the analysis of enzymatic activity during Enzyme Practical I: Effect of time, pH and temperature on the activity of acid phosphatase and Enzyme Practical II: Kinetics, effect of varying substrate concentration on enzyme activity was the addition of NaOH to your samples. Provide two reasons why you added NaOH and explain the molecular effects that NaOH has in each case!
What is the effect of (i) translation and (ii) rotation of an object on its Fourier...
What is the effect of (i) translation and (ii) rotation of an object on its Fourier transform?
According to the 'over justification effect', what is the consequence of rewarding children for an activity...
According to the 'over justification effect', what is the consequence of rewarding children for an activity that they already enjoy? a. The reward causes the children to perform the activity more independently. b. the reward causes the children to enjoy the activity less c. the reward causes the children to enjoy the activity even more d. the reward causes children to invest more time and effort in the activity
What is the goal of a phase II clinical trial? A. Demonstrate activity of the intervention....
What is the goal of a phase II clinical trial? A. Demonstrate activity of the intervention. B. Assess Drug-food interactions. C. Determine the maximum tolerated dose. D. Provide human pharmacokinetics data.
2b) Write a short conclusion about the effect of pH on enzyme activity. Include in your...
2b) Write a short conclusion about the effect of pH on enzyme activity. Include in your answer i) a concise (!) description of the trend observed in the graph shown in Enzyme Practical I: Effect of time, pH and temperature on the activity of acid phosphatase 2a) and ii) explain this trend using the language presented in this unit and your biochemical knowledge of enzymes and reactions. Total word count must be less than 100 (1.5 mark)
Question 1)i) Write a short conclusion about the effect of pH on enzyme activity. Include in...
Question 1)i) Write a short conclusion about the effect of pH on enzyme activity. Include in your answer i) a concise (!) description of the trend observed in the graph shown in Enzyme Practical I: Effect of time, pH and temperature on the activity of acid phosphatase? ii) explain this trend using the language presented in this unit and your biochemical knowledge of enzymes and reactions. Total word count must be less than 100
What effect would a mutation preventing the activity adenylate cyclase have on liver cells?
What effect would a mutation preventing the activity adenylate cyclase have on liver cells?
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT