Question

11. Assuming that there is a mutation such that the new sequence would be: DNA: TTAGCTAGATAAAACCGGCCCACGTTCTTACTTTT...

11. Assuming that there is a mutation such that the new sequence would be:

DNA: TTAGCTAGATAAAACCGGCCCACGTTCTTACTTTT

What would be the effect on the sequences of the mRNA & tRNA & the final protein’s sequence?

mRNA:

transcribe mRNA using the following from the DNA string: T=A, A=U, G & C pair

tRNA:

transcribe tRNA using the following from the mRNA string: A & U pair, G & C pair

Protein:

using the Condon chart and the mRNA strand. What I do with it if there is a stop?

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Answer #1

DNA: TTAGCTAGATAAAACCGGCCCACGTTCTTACTT TT.

Based on the given sequence, the mRNA, tRNA anti codons and Amino acid sequences will be as follows:

mRNA codons : AAU CGA UCU AUU UUG GCC GGG UGC AAG AAU GAA AA

tRNA anticodons : UUA GCU AGA UAA AAC CGG CCC ACG UUC UUA CUU UU

Amino acid sequences : Asn - Arg - Ser - Ile - Leu - Ala - Gly - Cys - Lys - Asn - Glu

If a new Stop codon is formed due to mutations in a DNA sequence, it will affect the structure of that protein. Any codons after the stop codon will be ignored during protein synthesis. These types of mutations are called Nonsense mutations.

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