Andy is studying the use of nitrate as a nitrogen source for biosynthesis. He is trying to identify the genes that are specifically involved in assimilation of nitrogen from nitrate. He made some mutants and now needs to identify the mutants of interest.
a.) Using minimal media, what nitrogen source should be used in the plate that will allow all mutants to grow?
b.) Using minimal media, what nitrogen source should be used in the plate that will prevent the mutants of interest from growing?
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