Question

4.1. Which of the following techniques would be best suited to determine where a particular protein is present in an embryo?

a) Chip-Seq

b) RNAseq

c) Immunostaining

d) Morpholino injection

4.2. Which of the following techniques is best suited to determine the phenotype of an embryo in the absence of a particular transcript?

a) Chip-Seq

b) RNAseq

c) Immunostaining

d) Morpholino injection

Answer 1

Answer :

4.1)

Correct choice is C

Immunostaining

Immunostaining in biochemistry is an antibody based method to detect a specific protein in a sample.

Chip seq is a method to understand protein interaction with DNA.

RNA seq is to reveal presence and quantity of RNA.

Morpholino injection is used to modify gene expression.

4.2)

Correct choice is A.

In absence of a transcript best was to understand phenotype is to study protein DNA interactions.

This can be done by Chip seq.

As its not transcribed no need for RNA seq.

As we dont know protein or phenotype no need of immunostaining.

Morpholino injection is to modify gene expression.