Question

32Some of the bonds can be disrupted upon protein denaturation. However, some of the proteins can refold and recover their native confirmation. The disruption of which bond will eliminate this possibility of refolding completely?

	a.	Hydrogen bond
	b.	Hydrophobic interaction
	c.	Disulfide linkage
	d.	Peptide bond

40If we snap-freeze the moment, in which tissue/organ do you expect to see a higher proportion of hemoglobin in T-state?

	a.	Skeletal muscle blood vessels during heavy workout
	b.	Pulmonary artery bloodstream during cardio
	c.	Bloodstream in aorta at rest
	d.	Capillaries in the lungs

2,3 – Bisphosphoglycerate (2,3 – BPG) in context of hemoglobin is:

	a.	Allosteric effector
	b.	Competitive inhibitor
	c.	Catalyst
	d.	Cofactor

Answer 1

32. (d) Peptide bond

When a protein is denatured, secondary and tertiary structures are altered but the peptide bonds of the primary structure between the amino acids are left intact. since all structural levels of the protein determine its funcion, the protein can no longer perform its function once it has been denatured.

40.(a) Skeletal muscle blood vessels during heavy workouts

(a) Allosteric effector

2,3-bisphosphoglycerate, is an allosteric effector of haemoglobin that regulates the affinity of hemoglobin for oxygen and facilitates the release of oxygen to the tissues.