For each of the following genotypes associated with the lacoperon in E. coli, indicate with a "+" or a "−" whether functional β-galactosidase would be produced in the following table:
β-galactosidase production
No Lactose With Lactose
I+ = normal repressor
I- = mutant repressor
Is = mutant repressor
O+ = normal operator
Oc = constitutive operator
(1 Point) Is Oc Z-/F'Is O+ Z+
β-galactosidase production
No Lactose With Lactose
A. + +
B. - -
C. + -
D. - +
Answer=(B) - -
Explanation-
In Merodiploid, strain 1 has mutant Beta-galactosidase (Z-) but strain 2 has functional Beta-galactosidase (Z+).
LacIs makes dominant repressor which is not bind to Inducer(allolactose) so this active repressor binds to normal operator(O+) region so RNA polymerase can't bind at promoter region so transcription of Beta-galactosidase (Z+) is inhibited in both condition (lactose present and lactase absent).
Rest all the options are incorrect regarding production of Beta Galactosidase (Z+).
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