Question

For each of the following genotypes associated with the lacoperon in E. coli, indicate with a...

For each of the following genotypes associated with the lacoperon in E. coli, indicate with a "+" or a "−" whether functional β-galactosidase would be produced in the following table:

β-galactosidase production

No Lactose     With Lactose

I+ = normal repressor

I- = mutant repressor

Is = mutant repressor

O+ = normal operator

Oc = constitutive operator

(1 Point)  Is Oc Z-/F'Is O+ Z+      

      β-galactosidase production

      No Lactose     With Lactose

A. + +

B. - -

C. + -

D. - +

Homework Answers

Answer #1

Answer=(B) - -

Explanation-

In Merodiploid, strain 1 has mutant Beta-galactosidase (Z-) but strain 2 has functional Beta-galactosidase (Z+).

LacIs makes dominant repressor which is not bind to Inducer(allolactose) so this active repressor binds to normal operator(O+) region so RNA polymerase can't bind at promoter region so transcription of Beta-galactosidase (Z+) is inhibited in both condition (lactose present and lactase absent).

Rest all the options are incorrect regarding production of Beta Galactosidase (Z+).

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region...
The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region containing the lac operon in E,coli is given, and the other fragment is from a plasmid carrying another lac operon from another source. The two are separated by a slash (/). The possible answers indicate with a ʺ+ʺ or a ʺ-ʺ whether β-galactosidase would be expected to be produced at induced levels under two circumstances: 1) first in the absence of lactose and 2)...
You isolated a mutant strain of E. coli in which the lac operon is constitutively expressed....
You isolated a mutant strain of E. coli in which the lac operon is constitutively expressed. To understand the nature of this defect, you created a partial diploid by introducing a plasmid containing a normal lacI gene (i.e. lacI+) and Z-. You then compared the mutant strain and the partial diploid with regard to their β-galactosidase activities in the presence and absence of lactose. You obtain the following results:                Lactose                        β-galactosidase expression Mutant                                     No                               High (constitutive expression) Mutant                                     Yes                             ...
For this question, we focus on four parts: I, the gene that produces the repressor protein;...
For this question, we focus on four parts: I, the gene that produces the repressor protein; P, the promoter region where RNA polymerase binds to initiate transcription; O, the Operator site, where the repressor binds. When the repressor binds, it prevents transcription; and ZYA, the three structural genes, all of which are required for the successful utilization of lactose as a food source. In the left column of the table below, we indicate the genetic composition at the lac operon...
The map of the lac operon and repressor gene is presented    here:                             &nbs
The map of the lac operon and repressor gene is presented    here:                                                 I     POZYA P is the promoter; O is the operator; Z encodes ß-galactosidase; Y encodes lactose permease; A encodes a transacetylase. The genotypes of merodiploids are shown below.  For each one, choose the correct phenotype from the table below.  (The same phenotype can be chosen for more than one genotype) PHENOTYPE                          ß-Galactosidase             No lactose                                with lactose A)                    -                                               + B)                    -                                               - C)                    +                                              + D)                    +                                              - I--P+OCZ+/I+P--O+Z-- Select one: a. A b. B c. C d. D The...
1.    The lac operon in E. coli comprises three structural genes, lac Z, lacY, and lacA....
1.    The lac operon in E. coli comprises three structural genes, lac Z, lacY, and lacA. It is regulated by both positive and negative mechanisms, involving binding of both a repressor protein and the catabolite activator protein (CAP). Transcription of the operon gives a polycistronic mRNA encoding the three enzymes. Under the following nutritional conditions, indicate whether or not the repressor and the CAP are bound by drawing them in on the appropriate regions of the operon. Indicate, as well,...
You are studying an E. coli strain with the following genotype: I + P+ Oc Z-...
You are studying an E. coli strain with the following genotype: I + P+ Oc Z- Y+ / F' I + P+ O+ Z+ Y- The lacZ mutation is a nonsense mutation. Which statement best describes its mutant phenotype? a. It will produce ß-galactosidase and permease in the presence or absence of inducer. b. It will produce ß-galactosidase in the presence or absence of inducer but will never produce permease. c. It will produce permease in the presence or absence...
Shown in the answers are four E. coli partial diploids. They each were assayed for production...
Shown in the answers are four E. coli partial diploids. They each were assayed for production of the Z and Y genes (ß-galactosidase and lactose permease, respectively) in the absence or presence of lactose. Which strain will not show wild-type regulation of lac permease enzyme activity encoded by Y? A.  isp + o +z + y -/ i -p + o +z - y + B.  i+p + o cz + y –/ i –p + o +z –y + C.  i+p –...
Gene expression includes transcription and translation to produce a protein product.  Below is a list of events...
Gene expression includes transcription and translation to produce a protein product.  Below is a list of events that occur during eukaryotic gene expression.  Among the events listed, which event occurs first?   Select one: a. binding of the small ribosomal subunit to the mRNA b. splicing out of the introns from mRNA c. binding of release factor to the mRNA d. binding of a charged tRNA to the first AUG codon e. peptide bond formed to the initiator methionine The map of the...
If E. coli were subjected to high levels of lactose and low levels of glucose, which...
If E. coli were subjected to high levels of lactose and low levels of glucose, which of the following conditions would apply? Y = yes, N = no A) lac repressor would be bound to the operator of the lac operon promoter B) CAP protein would be bound to the CAP binding site adjacent to the lac operon promoter C) cAMP levels would be low D) the lac operon would be actively expressing the operon into separate mRNA molecules needed...
Genetics: Upcoming exam, want to be prepared knowing the following questions. Make sure to answer with...
Genetics: Upcoming exam, want to be prepared knowing the following questions. Make sure to answer with one letter only. THANK YOU FOR YOUR HELP. 1. Recombination in individuals heterozygous for a ______ can produce chromosome containing two centromeres. a. inversion b. translocation c. duplication d. deletion e. RFLP 2. An E.coli strain possesses the following phenotype F(P+)/I^-P^cO^+Z^+Y^+ where I^- is a mutation that eliminates production of the repressor and P^c eliminates the ability of the polymerase to bind the promoter....