Question

1) Why do we need a standard in our ELISA experiment?

A. To create a stand curve

B. To ensure a high detection signal

C. To validate the false negative

D. To avoid a false positive

2) What is the purpose of agitating an ELISA plate?

A. optimizing the signal-to-noise ratio

B. Reducing the washing time

C. Increase the rate of binding

D. Prolong the incubation time

Answer 1

1) A OPTION IS CORRECT.

To create a stand curve we need a standard in our ELISA experiment. Becausehe the standard curve is prepared by serial dilutions of the standard. these curve used to determine the value of an unknown quantity from one that is more easily measured.  

B option is incorrect because substrate used for ensure a high detection signal.

2) B option is correct.

Agitating an ELISA plate reducing the washing time. Reason behind this Agitating/shaking remove nonspecific bindings and extra material from the plate.

D option is incorrect because Agitating shorten the incubation time.

A option is incorrect BECAUSE affinity-purified antibodies use for optimizing the signal-to-noise ratio.