Question

What are restriction endonucleases (RE) used for?

What are restriction endonucleases (RE) used for?

Homework Answers

Answer #1

Aeber, Nathan and Smith got Nobel prize for this 1978.

Restriction endonuclease is the enzyme which cut the double stranded helix (DNA) from the specific sites and also know as molecular scissor os biological scissors.

The single stranded free ends are called sticky ends which can be joined end to end by enzyme DNA ligase.

This enzyme have a key role in genetic engineering. The recognize the base sequence at palindrome sites in DNA duplex and cut its strands. For example Hin d ||| from heamophilus influenza Rd have pallandromic base sequence is 5'-AAGCTT-3/3'-TTCGAA-'5.

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