Question

A plasmid was digested with restriction enzymes Bam HI and Eco RV. The band sizes are given below. Use the data to construct a restriction map of the plasmid.

          Bam HI:       0.4 kb, 1.2 kb, 1.8 kb, and 2.2 kb

          Eco RV:       1.4 kb and 4.2 kb

          Bam H+ EcoRV:    0.3 kb, 0.4 kb, 0.5 kb, 1.2 kb, 1.4 kb, and 1.8 kb

Answer 1

In the question, you have to draw a restriction map. Restriction map is a map of chromosome which shows relative position of restriction sites for different restriction enzymes.

Restriction enzymes are the enzymes which cut DNA at a specific location called as restriction site.

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