1. COP II is a coatomer, a type of vesicle coat protein that transports proteins from the rough endoplasmic reticulum to the Golgi apparatus. Therefore, mutation in COP II protein will hamper the transport of proteins from the ER i.e. endoplasmic reticulum to the Golgi. Thus, the correct answer is budding vesicles from the ER.
2. The most important requirements to import proteins from cytoplasm to nucleus and peroxisome are: target sequences and protein translocator. Thus, the correct answers are protein translocator and signal sequence.
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