Question

A peptide of five amino acids contains one serine, one asparagine, one lysine and two aspartic acids. At pH 7.4, this peptide would have:

a) how many positive charge(s)

b) how many negative charge(s)

c) what is the overall net charge?

In a lab you are asked to pipette 130 mL using a pipette with a range of 20-200 mL. This pipette has recently been calibrated so is functioning correctly. You correctly move the dial of the pipette to 130 mL. On your first attempt to dispense 130 mL, you realise that the volume expelled was only 105 mL. On the second occasion it was 170 mL. Give explanations to what operator errors could have led to these outcomes?

Answer 1

Aa per policy question 1 is solved considering subparts a, b and c and Answer is attached .