Question

Describe the synthesis of leading strand during DNA replication. Assume the replication bubbled is open and...

Describe the synthesis of leading strand during DNA replication. Assume the replication bubbled is open and helicase has already opened the strand.

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Answer #1

Here prokaryotic replication is discussed

As replication fork is already is formed, so process continue with chain elongation.

Chain elongation: DNA chain elongation is catlyzed by enzyme DNA polymerase III using dNTPs (dATP, dCTP,dTTP, dGTP).

Excision of RNA primers and DNA replacement: Excision of RNA primer is carried out by DNA polymerase I by its exonuclease activity (5'->3').

Ligation: Ligation is carried out by enzyme DNA Ligase, it make phosphodiester bond between DNA fragment synthesized and DNA fragments came as replacement of RNA primers.

Termination: Replication halts at Ter site present in organism DNA sequence ( E.coli) with help of tus protein a product of Tus gene. It binds to Ter site and prevents strand displacement.

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