Question

5' AAGGCAAUAGCCAUG 3' 1. Give an example of single base pair substitution that could cause a...

5' AAGGCAAUAGCCAUG 3'

1. Give an example of single base pair substitution that could cause a truncation in this peptide.

2. Write down the sequence of the anticodon in the tRNA that would bind to the fourth codon in the mRNA

3. Write down a possible miRNA that could regulate the expression of this gene.

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Homework Answers

Answer #1

1) When there is found stop codon in the mRNA after substitutes, then protein is truncated.

The given mRNA sequence in the form of codon is:

5' AAG GCA AUA GCC AUG 3'

The A to U substitution occurs in :

5' UAG GCA AUA GCC AUG 3'

The UAG acts as stop codon.

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2) the sequence of tRNA is complementary to the the mRNA.

The tRNA sequence at fourth codon is: 3' CGG 5'

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3) The miRNA is complementary to the given mRNA sequence.

The sequence of mRNA is :

3' UUCCGUUAUCGGUAC 5'

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