Question

when a researched labels cDNA of the gene of interest, and uses it as a probe to detect mRNA in an organism, the researcher is carrying out the procedure

GFP fusion gene production

polypeptide purification

antibody targeting

RNA seq

RNA in situ hybridization

Answer 1

The correct answer is- e. RNA in situ hybridization

RNA in situ hybridization(ISH) particularly uses probe sequences of CDNA, RNA. These sequences are tagged with fluorescent compounds and radioisotopes. The important steps of ISH are as follows. At first, denaturation takes place and the probe of the complementary sequence is hybridized. After that renaturation of sequence occurs which is called reannealing. The complementary sequences of the probe bind at the target site. Counter staining helps to identify the hybridized site. E.g. FISH( Fluorescence In situ hybridization)