Question

24. Gene reporter assays using GFP has the following advantage(s) over using the GUS system:

• all of the above characteristics
• it is a non-destructive assay
• expression can be visualised by a simple fluorescence microscope
• GFP expression is instantaneous

Answer 1

24) Gene reporter assays using GFP has the following advantage(s) over using the GUS system :-

(a) All of the above characteristics

GFP = Green Fluorescent Protein

GUS = - glucuronidase

Gene reporter assays using GFP is a non-destructive whereas GUS is a destructive in nature due to histochemical staining and destaining procedures.

The expression using GFP can be easily visualised by a simple Fluorescence Microscope. The visualization is non-invasive and requires illumination of blue light only. High resolution visualisation is required in case of GUS.

GFP expression is instantaneous whereas GUS expression takes time.

Since all are correct statements are correct.

So the answer is (a) All of the above characteristics.