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4. The most important component of the protoplasting solution which ensures protoplast survival is: Cellulase Hemicellulase...

4. The most important component of the protoplasting solution which ensures protoplast survival is:

  • Cellulase
  • Hemicellulase
  • Hormones
  • MS basal salts
  • Mannitol
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Answer #1

Answer 4) option. Mannitol
The most important component of the protoplasting solution which ensures protoplast survival is Mannitol.
Normally cell wall is important for survival of cell as they stabilize the internal environment of the cell. But after the cell wall removal plasmalemma changes by either dilating or contracting due to changes in osmotic pressure. Therefore, Mannitol is added to maintain the osmotic pressure.

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