Question

9. The final stages of in vitro plant tissue culture, before the plants are transferred into the wild, is known as

• Embryogenesis
• Propagation
• None of the above
• Hardening

Answer 1

The answer is 4th option - Hardening

Hardening is the process of acclimatizing the in vitro grown plants in the artificial medium to the conditions in the natural environment. Before plants grown in an artificial culture are transferred to the natural environment, the method of preparing the invitro grown plants to grow in the outside natural environment by exposing them to natural conditions is known as hardening. The two stages of hardening are primary hardening and secondary hardening. In the process of hardening, the in vitro grown plants are kept in the greenhouse. It helps the plants to adjust to the new environment that has natural temperature, soil, light, and climate. It helps the plants grown in the artificial medium to survive in the natural soil

Therefore, the correct option is hardening