Question

If your 1:100 dilution of your extract gave an Abs595 reading of 2.6, a reading that falls above the linear range of your standard curve, what might you do? If your 1:100 dilution gave a reading of 0.003, a reading that falls below the linear range of your standard curve, what might you do?

Answer 1

In case of sample reading exceeding the standard curve, it should be further diluted to make the concentration in the range of standard curve. Also, in case when concentration of of sample is very low and the reading lying outside the standard curve, the curve should be recreated by including known concentration lower than the sample concentration.

This is because extrapolation may result in erroneous results. Extrapolation of a standard curve is not always true because at very high or low value the characteristics of curve may change. That is why only intrapolation is recommended.