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If you forgot to add DNA ligase to the ligation mixture in the lab, what could...

If you forgot to add DNA ligase to the ligation mixture in the lab, what could you expect to observe on the AMP-X-gal plate?

a. White colonies only

b. No bacterial growth

c. Blue and white colonies

d. Blue colonies only

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Answer #1

Answer- d. Blue colonies only

This is because this procedure is blue-white screen. In this, recombinant bacteria, with the DNA portion of interest inserted in it using vector, are detected along non-recombinant bacteria, using different coloured colonies.

The cells transformed with recombinant DNA form white colonies, while cells with non-recombinant plasmids form blue colonies.

Now, to be able to take up the recombinant DNA, it needs to be ligated into the host genome; in absence of ligase, this won't happen and all the cells will have non-recombinant plasmids only. Hence there will be blue colonies only.

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