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In chicken, protein factor GATA-1 specifically recognizes the WGATAR sequence in the close promoter of the...

In chicken, protein factor GATA-1 specifically recognizes the WGATAR sequence in the close promoter of the globin gene in red blood cells regulating its expression. How would you study if there are other genes subject to the same regulation?

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Answer #1

The regulation of proteins or any specific enzyme is occured by specific gen,but some times different genome also translates same protein or enzymes.It really adifficult task to predict that gene location,but there are few technique that we can use.

  • We can supress that specific gene and then if the same protein factor is then still being expressed then it is sure that there are other genes too which are responsible for expression of the protein factor.
  • Another technique is by using fluroscent dye-if we dye the gene sequence by using a fluroscent dye and if the protein does not show that specific fluroscent dye then it is confirmed that the protein is translated from from another sequence too.
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