Question

What is the purpose of DNA electrophoresis?

A) detect differences in DNA samples

B) create a buffer with various DNA samples

C) analyze the electrical charge of different DNA samples

D) produce DNA fragments of different sizes

Answer 1

Option A-Detect differences in DNA samples.

Electrophoresis methods like gel electrophoresis is a method used to seperate different DNA molecules based on their Charge.

DNA is a negatively charged molecule.

In Electrophoresis ...DNA Samples are placed at one end (Near -ve Electrode)and Electric current is Applied .So the negatively charge DNA molecules get moved from the area near -ve Electrode to area near Positive Electrode.

But this movement depends on the Size of the DNA molecules.Smaller DNA moves more and reaches more close towards the Positive electrode.While Large DNA molecules move less.

** This is used to seperate DNA molecules based on their size & not used to produce DNA Fragments.