Question

Transposon mutagenesis lab A bacterial cell contains pRL27, but the cell does not contain the pir...


Transposon mutagenesis lab

A bacterial cell contains pRL27, but the cell does not contain the pir gene. If this bacterial cell was grown in LB broth, then describe the population of cells generated in the resulting culture and how they were generated? Be specific.

Homework Answers

Answer #1

PIR protein is essentially required for the replication of plasmid. Therefore, if the cell lack the PIR gene pRL27 plasmid will not be able to replicate with in the cell because pRL27 plasmid is PIR protein dependent a conjugative plasmid. The pRL27 plasmid contains antibiotic resistance gene such as kanamycin (Kan) and highly active Tn5-transposase gene (TNP). Therefore, when the transformed bacteria cell pRL27 plasmid were allow to grow in a broth (selection medium with kanamycin) the bacterial population will consistently decline because in the absence of PIR gene the pRL27 plasmid will not replicate and the cell won’t be kanamycin resistance.

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
QUESTION 1 Why do non-fermenters form white/colorless colonies on MacConkey agar? a. They breakdown mannitol b....
QUESTION 1 Why do non-fermenters form white/colorless colonies on MacConkey agar? a. They breakdown mannitol b. They are gram positive c. They are coliform d. They do not breakdown lactose e. They are fecal coliform 5 points    QUESTION 2 Bacteria that can breakdown (ferment) lactose would show this result. a. Yellow color change on a mannitol salt agar b. Yellow color change on a lactose phenol red broth tube c. Green/brown color change on blood agar d. Red color...
1. Which sentence best defines signal transduction in a biochemical context? a. Alteration of cell functions...
1. Which sentence best defines signal transduction in a biochemical context? a. Alteration of cell functions in response to the environment b. Generation of a chemical change in response to information c. Induction of cell death upon nutrient starvation d. Responses to stimuli 2. Which statement is correct? a. G protein-coupled receptors produce second messengers indirectly while receptor enzymes catalyse second messenger generation b. Second messengers stimulate the activity of G protein-coupled receptors. c. Receptor enzymes produce second messengers through...
The picture shown below shows variation among three individuals with respect to 4 nucleotides - AGAT....
The picture shown below shows variation among three individuals with respect to 4 nucleotides - AGAT. What do you think what type of variation is this? AGAT different repeat numbers in different individuals .png Minisatellite Single nucleotide polymorphism Short Tandem Repeats Which of the following statements is TRUE about DNA matching? Typical difference between the genomes of human beings and Chimpanzees is estimated to be 25 % Typical difference between the genomes of human beings and Drosophila is   estimated to...
READ THE CASE STUDY AND ANSWER THE FOLLOWING QUESTIONS 2nd CASE: An Unexplained Death A 65-year-old...
READ THE CASE STUDY AND ANSWER THE FOLLOWING QUESTIONS 2nd CASE: An Unexplained Death A 65-year-old man of Scandinavian descent was rushed to the Emergency Room of your local hospital after a family member discovered him unconscious in his home. The woman who dialed “911” told the dispatcher that the man, her brother, was the local librarian of the past 10 years and had no spouse or children. She reported that they had spoken the day before, and he had...
Assignment: What are the main arguments in the article? Please answer within 5 hours. It is...
Assignment: What are the main arguments in the article? Please answer within 5 hours. It is extremely urgent!!!!!!!!!!!!!!!!!!!!!!!! --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- BIOETHICS. Bioethics as a field is relatively new, emerging only in the late 1960s, though many of the questions it addresses are as old as medicine itself. When Hippocrates wrote his now famous dictum Primum non nocere (First, do no harm), he was grappling with one of the core issues still facing human medicine, namely, the role and duty of the...
What topics are covered in the following article? Please answer within 5 hours. It is extremely...
What topics are covered in the following article? Please answer within 5 hours. It is extremely urgent!!!!!!!!!!!!!!!!!!!!!!!! --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- BIOETHICS. Bioethics as a field is relatively new, emerging only in the late 1960s, though many of the questions it addresses are as old as medicine itself. When Hippocrates wrote his now famous dictum Primum non nocere (First, do no harm), he was grappling with one of the core issues still facing human medicine, namely, the role and duty of the physician....