Question

Describe the steps and their purpose in the thermal cycle of PCR.

Answer 1

Steps of thermal cycle of PCR :

Thermal cycling is a process where polymerase chain reaction involves in heating and cooling by thermocycler machine .It has 20-40 series of repeated cycle. In this cycle ,each cycle there are 3 steps found. The steps are given below .....

• Denaturation - here, double stranded template DNA heated into 94°C and then separate into two single strands .
• Annealing - here, lower temperature that is 50° C enable DNA primer to attaching thr template DNA.
• Elongation - here, new strand DNA is made by raising the temperature of 72°C. Purpose of thermal cycle of PCR -

Thermocycler which is a laboratory apparatus, used to amplifying the DNA segments in the PCR process.It also animates other temperature sensitive reactions.