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Describe, in detail, how you would obtain a gene from a tomato plant and express this...

Describe, in detail, how you would obtain a gene from a tomato plant and express this protein in bacterial cells. BamHI restriction sites at either end surround the tomato gene of interest. You may use any vector of your own design in the process.

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The gene of interest can be amplified using the primer containing (forward primer) NheI and (Reverse primer ) BamHI (if they does not digest gene).amplified product can be purified and then digested with the selected enzyme same time vector containing this site also can be digested (pET 28a for expression).again purification can be performed for digested product and then ligation can be performed using ligase. Ligated product can be transferred into E coli cells and plasmid can be procured. And then this plasmin can be transformed in to expression E coli strain and through IPTG induction protein can be induced and then it can purified after lysis of the cells through His tag using Ni-NTA column.    

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