Question

PGI uses a histidine and a glutamate residue as acid-base catalysts during the catalytic cycle. Draw a single mechanism for the reversible PGI-catalyzed reaction. Indicate the flow of electrons for the forward (glycolytic) direction using solid arrows and that for the reverse (gluconeogenesis) direction using dashed arrows

Answer 1

Phosphoglucose isomerase (PGI) plays a central role in the metabolism of phosphorylated sugars in the body. It basically catalyzes the conversion of Glucose-6-PO4 to fructose-6-PO4 during the glycolysis pathway.

Literature indicates that PGI contains histidine and glutamate residues in its active site for binding.

NAD+ accept electron to convert into NADH.