Question

Your goal is to make the cells as green as possible by maximizing production of GFP. What change can you make to each of the following to maximize expression of the GFP gene?

Positive Transcription Factor Concentration:

mRNA Destroyer (exonuclease) Concentration:

Positive Transcription Factor Affinity for the Gene Regulatory Sequence:

RNA Polymerase Affinity for the Gene Regulatory Sequence:

Protein Degradation:

Answer 1

Ans-

A) Increase

Increasing the concentration of positive transcription factor will increase the rate of transcription of specified gene.

B) Decrease

Decreasing the concentration of exonuclease will prevent mRNA from degradation and hence, promoing translation of mRNA and therefore, larger protein will be expressed.

C) Increase

Increasing the affinity of positive transcription factor for regulatory sequence will increase production of mRNA transcripts and hence, increased expression of protein.

D) Increase

Increasing the affinity of RNA polymerase for regulatory gene will produce more mRNA transcripts and hence, increased protein expression.

E) Decrease

For high expression of protein, it is essential for prevent its degradation.