Question

Why does RNA polymerase II get phosphorylated by TFIIH?

Answer 1

The eukaryotic RNA polymerase is of mainly three types RNA polymerase 1, RNA polymerase ll, and RNA polymerase lll. Out of these the RNA polymerase ll is very important. The RNA polymerase ll synthesizes mRNA. It also transcribes small nuclear RNAs. For eukaryotes an important transcription factor needed for the pre-initiation of the complexes is TFllH. This is due to the ATP dependent helicase, enzyme that phosphorylates the c- terminal of RNA polymerase ll. This c-terminal domain causes the elongation of the RNAPll. The THllF and RNAP maintains the transcription bubble and the TFllD bonds with TATA box. The TFllB binds to TFllF and other Tfs binds to form transcription pre- initiation complexes. Due to the TFllH helicase seperates the DNA strands and the RNA polymerase ll transcription starts.