5’…TAC ACC GAT GGA TAA GTC…3’ DNA Coding strand
3’…ATG TGG CTA CCT ATT CAG…5’ DNA template strand
Use the information above to answer the following questions.
The two duoble stranded daughter DNAs are:
1. 5'...TAC ACC GAT GGA TAA GTC...3'
3'...ATG TGG CTA CCT ATT CAG... 5'
2. 5'...GAC TTA TCC ATC GGT GTA...3'
3'...CTG AAT AGG TAG CCA CAT... 5'
The mRNA molecule transcribed from the given template is as:
5'... UAC ACC GAU GGA UAA GUC... 3'
The sequence of the tRNA which has anti-codons for the above mRNA codons is as:
5'...GAC UUA UCC AUC GGU GUA.... 3'
The sequence of the amino acids translated from the mRNA transcript is as:
Only four out of the six codons will be translated because the fifth codon UAA is a STOP CODON.
If the second A is replaced by T in the template then Tlthe third codon, GAU will become GAA.
The amino acid in place if Asp will be replaced by Glu.
The property of the protein does not change much because one acidic amino acid is replaced by another acidic amino acid.
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