Question

Suppose you are a student performing research in a molecular genetics lab. Your project involves PCR amplification of your own Hemoglobin gene. One day, you decide to check your Huntington’s disease risk by amplifying your own HTT gene. (PSA: This is just a test question! Genetic counselors recommend consulting a healthcare provider for any DNA testing.)

What component must be changed in the PCR to check for the risk of Huntington’s disease?

a) The DNA template

b)The DNA polymerase

c) The nucleotides

d) The primers

e)The reaction buffer

Answer 1

the correct option should be C ...The Huntington's Disease (HD) Collaborative Research Group has as of late distributed the succession of another cDNA, IT15, containing a polymorphic trinucleotide (CAG)n rehash that is extended and flimsy on HD chromosomes. There is a connection between's the recurrent size and the period of beginning of manifestations. The proposed polymerase chain response (PCR) examine of the (CAG)n rehash requires bizarre response parts and groundwork focuses and the utilization of 5% polyacrylamide sequencing gels to determine the intensification items....This gives better resolution of the (CAG)n expansion observed on HD chromosomes by acrylamide gel electrophoresis and allows sufficient product to be obtained to perform assays using agarose gels. This will allow diagnostic labs to do rapid and accurate presymptomatic testing of HD in high risk families.