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Describe a research plan you would use to identify the molecular mechanisms for its persistence and...

Describe a research plan you would use to identify the molecular mechanisms for its persistence and recurrent cause of DFI disease.(Staphylococcus aureus in involved in this infection)

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Answer #1

Mechanisms for identification of DFI(Diabetic foot ulcers) includes

Culture based methods for identification of DFI e.Gg. Wound biopsy culture

16S pyrosequencing based methods to identify the variety of microbiota associated with this.

Intracellular persistance assay can be done -For this cell lines can be used such as Murine macrophages. It can be infected with strains of S.aureus and then different parameters. Post infection the persistence can be determined by several assays such as immunofluorecence microscopy bacteriocidal protection assay , phagocytosis assays.

Colocalisation experiments can also be performed using different endocytic markers Lamp, Rab7 .

Drug treatment such as drugs that blocks actin polymerisation of assembly and therefore the phagocytosis will reduce bacterial growth.

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