Protein, a long chain of amino acids present in different shape and size, they may be simple chain of amino acids in primary structure or highly coiled and complex in quaternary structure. Usually three-dimensional (3D) structure present in the tertiary structure of proteins, which exhibits different covalent bonds among amino acids like hydrogen or disulfide bonds but not peptide bonds. So basically in the 3D imaging through NMR, they tell us about the sequence of amino acids in the polypeptide chain Hence, based on this most relevant choice would be “A) sequence of amino acids in the polypeptide chain” as compared to rest choices, which is either partially correct or incorrect (or less important in the given situation).
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