Question

What is the difference between a site-specific endonuclease and a general endonuclease?

What is the difference between a site-specific endonuclease and a general endonuclease?

Homework Answers

Answer #1

A general endonuclease is an enzyme of the DNase family that cuts the DNA between two nucleotides. That is, it digests the phosphodiester bond between two nucleotides in a DNA. It is different from exonuclease which cut DNA from the ends.

A site specific restriction endonuclease is a special type of endonuclease. It has the additional feature of recognizing a particular sequence of nucleotides (known as restriction site) in the DNA and then cut or near that site. The recognition sites of site specific endonucleases are generally palindromic sequences, that is they are the same sequence on the complimentary strands but in opposite reading directions.

For example, GAATTC is the recognition site of the endonuclease EcoRI

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