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Do you expect to see phospholipase C activation (high IP1 concentrations) in eggs treated with A23187...

Do you expect to see phospholipase C activation (high IP1 concentrations) in eggs treated with A23187 when performing the ELISA test? (sea urchin experiment)

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Answer #1

The chemical A23187 is a calcium ionophore by nature which promotes channelization of calcium ions across the membrane, thus increasing the intracellular calcium content in the cells. Thus, application of this chemical to the eggs would enhance intracellular calcium content of the eggs. Further, the phospholipase C activation secondary to IP1 levels is a complex set of intracellular signalling mechanism which is up-regulated in the presence of large calcium pool.  

Hence, the information suggests that the ionophore would act as a positive regulator of calcium content in the eggs and hence upregulate the total phospholipase C activation. Consequently, a positive result is expected in the ELISA test in this condition.

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