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You streak a culture and find that the colonies in quadrant 4 are larger in diameter...

You streak a culture and find that the colonies in quadrant 4 are larger in diameter than the colonies in quadrant 2. Why might this be?

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Answer #1

In the quadrant streak method, four equally sized sections are streaked in an agar plate. Here, the plate is turned 180o in between streaks. It involves dragging of the bacteria from streak on one quadrant to the next quadrant. Therefore, the first quadrant will have a tight streak due to the higher number of bacteria and their growth. The bacterial density will be much lesser in quadrant four. Because of the less number of bacteria in quadrant four, the availability of the nutrients will be more in this quadrant and hence, the colonies in quadrant four are larger than in other quadrants.

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