Question

You are testing protein X and protein Y in a yeast two-hybrid assay that uses lacZ as a reporter gene. Production of a blue product signifies that:

• A. protein Y produced protein X

• B. protein X produced protein Y

• C. protein X does not interact with protein Y

• D. protein X interacts with protein

Answer 1

Ansd) protein X interacts with protein Y

Explaination:

Blue colour formation indicates the transcription of beta galactosidase which occur when both X and Y protein interact with each other. yeast two hybrid assay used to study protein -protein interaction in which proteins are called as bait and prey and transcription or expression of beta galactosidase occur when bait part of one protein ie, protein X fuses with prey part of another protein ie, proteinY to induce expression of beta galactosidase which is indicated by blue colonies in presence of X-gal.