Question

I have done these problems but am unsure if the answers I got were correct, Please show all steps so I can fully understand!

1.) How many **microliters** of original sample are
required to produce a final dilution of 10^{-3} in a total
volume of 1 mL? INFO: 1
microliter is 10^{-6} L or 10^{-3} mL.

2.) If you suspect your culture of bacteria has 240 x
10^{6} cells per mL, what would you want the final dilution
to be in order to end up with 30 cfu on a plate?

Express your answer as an exponent rounded to two decimal places. Eg. 10.01E-3, not 0.010011.

Answer #1

**1)**

Dilution = 1/1000

1 part in 1000, so volume (parts) in 1 mL,

= 1/1000 mL

= 10-3 mL

= 1 microliter

**2)**

you can do serial dilutions :

**1st tube :** 1/100 dilution

Concentration of cells will be : 240 × 104 cell/mL

**2nd tube :** 1/100 dilution

Concentration of cells : 240 × 102 cells/mL

**3rd tube :** 1/100 dilution

Concentration of cells would be : 240 cells/mL

**4th tube :** 1/2 dilution

Concentration of cells would be 120 cells/mL

**5th tube :** 1/2 dilution

Concentration of cells would be 60 cells/mL

**6th tube :** 1/2 dilution

Concentration of cells would be **30**
**cells/mL**

Total dilution of tube 6 :

= 1/100 × 1/100 × 1/100 × 1/2 × 1/2 × 1/2

= 1/8000000

I have done these problems but am unsure if the answers I got
were correct, Please show all steps so I can fully understand!
1.)
A 0.1 dilution is performed on a culture of bacteria in order to
perform viable plate counts. From the dilution,
*0.1 mL* of
solution is plated on solid media, and 154 colony forming units
grow on the plate. How many bacteria are in a single mL of the
original culture? Express your answer to two...

Question 4 please although I am unsure of my answers for the
rest as well :(
Here is the full list for background info.
Consider a significance test for a null hypothesis versus a
two-sided alternative. State all values of a standard normal test
statistic z that will give a result significant at the 10%
level but not at the 5% level of significance. (Sec. 6.2)
You perform 1,000 significance tests using α = 0.01.
Assuming that all the null...

C Programming:
I am working on the problem below and have got all of the code
down, except for one part I can't figure out.
The logic for the calculation where the the total at each shop
is giving after each iteration of the loop.
this is the formula I've got:
ingredientPrice += ingredient1;
It calculates the total for the first shop properly, but for the
shops that follow it gives a cumulative total rather than the
singular total for...

Before whoever decides to answer the following problems, please
DO NOT use excel. I have posted this question already, and the
expert answered using excel to solve this problem. I am not good
with excel at all so I did not understand what was given. Would
rather have the problem worked out showing the formula and work
broken down and written out. Thank you in advance.
You have been assigned the task of evaluating two mutually
exclusive projects with the...

Please answer the following Case
analysis questions
1-How is New Balance performing compared to its primary rivals?
How will the acquisition of Reebok by Adidas impact the structure
of the athletic shoe industry? Is this likely to be favorable or
unfavorable for New Balance?
2- What issues does New Balance management need to address?
3-What recommendations would you make to New Balance Management?
What does New Balance need to do to continue to be successful?
Should management continue to invest...

What tools could AA leaders have used to increase their
awareness of internal and external issues?
???ALASKA AIRLINES: NAVIGATING CHANGE
In the autumn of 2007, Alaska Airlines executives adjourned at
the end of a long and stressful day in the
midst of a multi-day strategic planning session. Most headed
outside to relax, unwind and enjoy a bonfire
on the shore of Semiahmoo Spit, outside the meeting venue in
Blaine, a seaport town in northwest
Washington state.
Meanwhile, several members of...

What role could the governance of ethics have played
if it had been in existence in the organization? Assess the
leadership of Enron from an ethical perspective.
THE FALL OF ENRON: A STAKEHOLDER FAILURE
Once upon a time, there was a gleaming headquarters
office tower in Houston, with a giant tilted "£"' in front, slowly
revolving in the Texas sun. The Enron Corporation, which once
ranked among the top Fortune 500 companies, collapsed in 2001 under
a mountain of debt...

ADVERTISEMENT

Get Answers For Free

Most questions answered within 1 hours.

ADVERTISEMENT

asked 32 minutes ago

asked 59 minutes ago

asked 1 hour ago

asked 1 hour ago

asked 1 hour ago

asked 1 hour ago

asked 1 hour ago

asked 1 hour ago

asked 1 hour ago

asked 1 hour ago

asked 1 hour ago

asked 1 hour ago