Question

How are you able to ensure that the E. coli you grew for an RNAi experiment...

How are you able to ensure that the E. coli you grew for an RNAi experiment contains the plasmid you ordered from the RNAi library?

Group of answer choices

Grow in presence of antibiotic

All bacteria will have it

Grow in presence of GFP

Use PAM sequence

Add IPTG to the culture

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Answer #1

Answers are "ALL BACTERIA WILL HAVE IT" as E.coli is known to have 2 plamsids. The E.coli grown from the rnai experiment are known for resistance to amphicillin thus "GROW IN PRESENCE OF ANTIBIOTIC".

Why not Grow in presence of GFP - green fluorescence protein, it is found in marine organisms mainly, thus it is not a choice fr determining, Use PAM sequence, it is used for the cut of Cripsr locus and the add IPTG that is used to express protein below 16°centigrade.

Thankyou..

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