Question

How are you able to ensure that the E. coli you grew for an RNAi experiment...

How are you able to ensure that the E. coli you grew for an RNAi experiment contains the plasmid you ordered from the RNAi library?

Group of answer choices

Grow in presence of antibiotic

All bacteria will have it

Grow in presence of GFP

Use PAM sequence

Add IPTG to the culture

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Answers are "ALL BACTERIA WILL HAVE IT" as E.coli is known to have 2 plamsids. The E.coli grown from the rnai experiment are known for resistance to amphicillin thus "GROW IN PRESENCE OF ANTIBIOTIC".

Why not Grow in presence of GFP - green fluorescence protein, it is found in marine organisms mainly, thus it is not a choice fr determining, Use PAM sequence, it is used for the cut of Cripsr locus and the add IPTG that is used to express protein below 16°centigrade.

Thankyou..

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
The genome of the bacteriophage M13 was modified to include a CRISPR-Cas 9 operon with the...
The genome of the bacteriophage M13 was modified to include a CRISPR-Cas 9 operon with the following features: The operon includes a gene coding for protein Cas 9, and a gene coding a sgRNA. The CRISPR-Ca9 operon is under the control of a bacterial constitutive promoter. The sgRNA gene includes a spacer flanked by two repeats. The sequence of the spacer matches a sequence of an antibiotic resistance gene present in an antibiotic resistant form of a bacteria. Bacteria containing...
8. You are preparing a 50mL culture of E. coli in LB broth containing the antibiotic...
8. You are preparing a 50mL culture of E. coli in LB broth containing the antibiotic apramycin. The culture media will also need to include L-arabinose to induce gene expression. Answer the following questions and show all work. a. How would you make 15mL of a 1M stock of L-Arabinose (MW = 150g/mol)? (1pt) b. How much L-arabinose (from the stock in 8a) do you add to make the final concentration of L-Arabinose in the culture 10mM (.010M)? (1pt) c....
Which experiment(s) do you suggest to determine whether your culture contains a bacteria or an archaea?...
Which experiment(s) do you suggest to determine whether your culture contains a bacteria or an archaea? analyze the cell wall composition all proposed answers are appropriate experiments look whether it has an exonuclease grow the cells at high salt concentration sequence the ssrRNA gene
1. Your family and friends ask you, “Is E. coli a pathogen?” What would you answer?...
1. Your family and friends ask you, “Is E. coli a pathogen?” What would you answer? which of the following sentences is not correct a. Some E. coli grow normally in the human intestine, producing vitamins, such as vitamin K b. E. coli, like other microbes can live symbiotically in our gut c. Other strains, such as E. coli O157:H7, cause serious illness. d. Location, location, location. If it stays where it should be no strain of E. coli is...
1. Currently you are studying threonine synthesis in E. coli. To find genes whose protein products...
1. Currently you are studying threonine synthesis in E. coli. To find genes whose protein products are important for threonine synthesis, you perform a mutagenesis to find mutants that require a source of threonine to grow. (a) Immediately after mutagenizing the bacteria, would you choose to grow the bacteria on rich media or on minimal media? Briefly explain your choice. Rich media. A mutant unable to make a particular amino acid will not be able to grown on media lacking...
QUESTION 1 Why do non-fermenters form white/colorless colonies on MacConkey agar? a. They breakdown mannitol b....
QUESTION 1 Why do non-fermenters form white/colorless colonies on MacConkey agar? a. They breakdown mannitol b. They are gram positive c. They are coliform d. They do not breakdown lactose e. They are fecal coliform 5 points    QUESTION 2 Bacteria that can breakdown (ferment) lactose would show this result. a. Yellow color change on a mannitol salt agar b. Yellow color change on a lactose phenol red broth tube c. Green/brown color change on blood agar d. Red color...
1. A patient suffers from a fungal infection, which medicine are you going to prescribe? a....
1. A patient suffers from a fungal infection, which medicine are you going to prescribe? a. daptomycin b. platensimycin c. tetracycline d. penicillin e. none of the above 2. How would hou induce antiobiotic production by a soil bacterium a. lower the pH b. higher the pH c. add more cells to the culture d. increase temperature e. decrease temperature 3. which experiment(s) do you suugest to determine whether your culture contains a bacteria or achae? a. analyze the cell...
Using the model proposed by Lafley and Charan, analyze how Apigee was able to drive innovation....
Using the model proposed by Lafley and Charan, analyze how Apigee was able to drive innovation. case:    W17400 APIGEE: PEOPLE MANAGEMENT PRACTICES AND THE CHALLENGE OF GROWTH Ranjeet Nambudiri, S. Ramnarayan, and Catherine Xavier wrote this case solely to provide material for class discussion. The authors do not intend to illustrate either effective or ineffective handling of a managerial situation. The authors may have disguised certain names and other identifying information to protect confidentiality. This publication may not be...
After reading the following article, how would you summarize it? What conclusions can be made about...
After reading the following article, how would you summarize it? What conclusions can be made about Amazon? Case 12: Amazon.com Inc.: Retailing Giant to High-Tech Player? (Internet Companies) Overview Founded by Jeff Bezos, online giant Amazon.com, Inc. (Amazon), was incorporated in the state of Washington in July 1994, and sold its first book in July 1995. In May 1997, Amazon (AMZN) completed its initial public offering and its common stock was listed on the NASDAQ Global Select Market. Amazon quickly...
Please answer the following Case analysis questions 1-How is New Balance performing compared to its primary...
Please answer the following Case analysis questions 1-How is New Balance performing compared to its primary rivals? How will the acquisition of Reebok by Adidas impact the structure of the athletic shoe industry? Is this likely to be favorable or unfavorable for New Balance? 2- What issues does New Balance management need to address? 3-What recommendations would you make to New Balance Management? What does New Balance need to do to continue to be successful? Should management continue to invest...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT