Question

If you had to make a buffer with a 250 mM solution of Tris-Base (FW= 121.14)...

If you had to make a buffer with a 250 mM solution of Tris-Base (FW= 121.14) in a volume of 600 mL, how many grams of Tris-Base will you need to weigh out?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

FW = 121.14 g/L

Desired volume of the solution = 600 ml = 0.6 L

Desired molarity of the solution = 250 mM = 0.25 M

Here, 121.14 g in 1 L will have a molarity of 1 M (or 1000 mM)

The equation is

Grams / desired volume of the solution in L = required molarity in mole/L x FW in g/L

Grams = desired volume of the solution in L x  required molarity in mole/L x FW in g/L

Substituting the equation with the values:

Grams = 0.6 x 0.25 x 121.14

= 18.171 g

Therefore, for making 250 mM of Tris-base in a volume of 600 mL, 18.171g of Tris-base is needed.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
*Calculate how many grams you need to make a 20mM buffer of Tris/ 1mM EDTA buffer?...
*Calculate how many grams you need to make a 20mM buffer of Tris/ 1mM EDTA buffer? (1360 mL final volume) Tris:121.14 g/mol. EDTA:292.24 g/mol
You need to make 50 mL of a 25 mM Tris buffer that has a pH...
You need to make 50 mL of a 25 mM Tris buffer that has a pH of 8.40. Show calculations for and describe how you would make it if you had access to: •A) Solid Tris acid (Tris HCl) and solid Tris base •B) Solid Tris acid and 1.0 M NaOH •C) Solid Tris base and 1.0 M HCl
TAE is a commonly used buffer for DNA el ectrophoresis. This buffer contains 40 mM Tris,...
TAE is a commonly used buffer for DNA el ectrophoresis. This buffer contains 40 mM Tris, 20 mM Acetate, 1 mM EDTA as the final concentrations. A 50X stock solution (50X the final concentration) is normally prepared. You have Tris powder (mol. wt. 121.14), glacial acetate acid (a liquid with a density of 1.05 g/mL and a mol. wt. of 60) and 0.5 M EDTA stock solution. You need to make up 1 Li ter of 50X TAE. How would...
To prepare 100 mL of 25 mM Tris buffer at pH 8.0, calculate the amount of...
To prepare 100 mL of 25 mM Tris buffer at pH 8.0, calculate the amount of Tris base and Tris·HCl needed to make the buffer. (Hint: find out the pKa of Tris·HCl and use the Henderson-Hasselbalch Equation.) Describe how do you want to make the solution, including how much water to add, how to adjust pH, etc.
How many mmols of Tris would you need to prepare 100 mL of a 100mM Tris...
How many mmols of Tris would you need to prepare 100 mL of a 100mM Tris solution? How many grams of Tris (MW 121.1) would you need ot prepare 100 mL of a 100 mM Tris solution?
you need 250ml of a 100 mM of tris buffer at pH of 8.25. the molecular...
you need 250ml of a 100 mM of tris buffer at pH of 8.25. the molecular weight of tris is 121.1 and its pKa is 8.06. A) how much tris do you need to weight out? B) you have 10 M HCL soulution, how much will you add to get the right pH?
If you dissolved had a 0.5M Tris buffer (Molecular weight is 121.1grams/mole) at pH 6.5 and...
If you dissolved had a 0.5M Tris buffer (Molecular weight is 121.1grams/mole) at pH 6.5 and needed to adjust this solution to pH 8.0 how many would you get this solution to a pH of 8.0? Indicate what you would need to add, HCl or NaOH and what volume of a 1M solution of HCl or NaOH you would need to add to obtain a final pH of 8.0? Assume here you will end with a 1L solution.
A buffer made of 250 mM sucrose and 50 mM sodium phosphate is used to homogenize...
A buffer made of 250 mM sucrose and 50 mM sodium phosphate is used to homogenize the tissues for this experiment. You have 2M sodium phosphate buffer and dry sucrose. How would you make 750 ml of the buffer? Show your calculations. (HINT: there is one piece of information that is NOT given that you need to find out in order to answer this)
To make up a solution of PBS, you need 15 mM Na2HPO4 (141.96 g/mol), 0.25M NaCl...
To make up a solution of PBS, you need 15 mM Na2HPO4 (141.96 g/mol), 0.25M NaCl (58.44 g/mol), and 2 mM KH2PO4 (136.09 g/mol). How many grams of each will you need to make up 600 mL of PBS?
Miniprep DNA Wash Buffer (PE) contains: 10 mM Tris-HCl pH 7.5 and 80% ethanol (v/v) Answer...
Miniprep DNA Wash Buffer (PE) contains: 10 mM Tris-HCl pH 7.5 and 80% ethanol (v/v) Answer the following: a. You are asked to prepare 80 mL of DNA Wash Buffer (PE). How many mL of 100% ethanol should be added, then the total volume adjusted to the 80 mL final volume?
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT