Question

Part A: Using a codon table, translate this section of mRNA

5’ ----CGGAUUCUCGGGAAC---- 3’

Part B:

A bacterium has this sequence for the same piece of mRNA:

5’ ----CGGAUUCACGGGAAC---- 3’

What type of mutation does this represent?

Explain the consequences of this mutation on the protein AND on the cell.

Answer 1

Part A- First we will translate the given section of mRNA

mRNA - 5’ ----CGGAUUCUCGGGAAC---- 3’

Amino acid sequence- 5' ---Arg Ile Leu Gly Asn --3'

Part B- Now we will translate the given sequence of mRNA present in bacteria and see what type of mutation is it

mRNA - 5’ ----CGGAUUCACGGGAAC---- 3’

Amino acid sequence - 5'---Arg Ile His Gly Asn---3'

So, as you can see initially the third amino acid was leucine, but it changed to histidine after mutation. It is a type of missense mutation as there is change in amino acid being coded after mutation. This would result in the formation of different protein after mutation.

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