Question

1. Describe the results of your BLAST search, including the breadth of organisms that appear to
have a PAX-6 homolog, and how many PAX-6 homologs appear to be present in the human genome.
2. Describe the closest human homolog. How long is the protein, and how similar is it to PAX-6 at
the protein level?
3. Describe the function and expression pattern of this protein. Where is it typically expressed?
What is it thought to do? What is its subcellular localization, and does this make sense in light
of its molecular function?
4. What functional domains are present in this protein? What are their proposed functions?
5. Of the four non-human homologs you entered into CLUSTALW, which is mos closely related to the
human protein? What data supports this hypothesis?

Answer 1

1. The human PAX6 locus encodes no less than three diverse protein isoforms, these being the standard PAX6, PAX6 (5a), and PAX6 (?PD).

2. Closest human homolog is mouse. Isoforms PAX6, PAX6 (5a), and PAX6 (?PD) molecular weights of 43, 33, or 32 kDa, mouse and human have identical AA arrangement.

3. It goes about as an "master control" gene for the advancement of eyes and other tactile organs, certain neural and epidermal tissues and different homologous structures, typically got from ectodermal tissues.

4. The trademark domain of Pax6 uses two , the matched space paired domain (PD), and the paired-type homeodomain (HD).

HD’s regulatory involvement in the formation of the lens and retina.

Control exhibited on the patterns of neurogenesis in brain development by PD.