Question

Calculate the resolving power of a microscope that uses a wavelength of light of 0.2 microns...

Calculate the resolving power of a microscope that uses a wavelength of light of 0.2 microns (micrometers) and has a numerical aperature of 1.6

Homework Answers

Answer #1

Wavelength () = 0.2 microns = metre = 0.0000002 m

Numerical aperture (NA) = 1.6

Smallest distinguishable distance (d) =

= 0.61 * 0.0000002 / 1.6

= m

= 76.25 nanometre

Resolving power = 1/d = 1.31 x 10^7

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